mouse bone marrow mesenchymal stem cells bmscs  (ATCC)

Journal: Journal of Nanobiotechnology

Article Title: Supramolecular delivery of senolytics enables targeted anti-senescence therapy and accelerated fracture healing

doi: 10.1186/s12951-026-04138-2

Figure Lengend Snippet: Asp 10 SAC4A enables hypoxia-activated senolysis and promotes osteogenic differentiation in vitro. A ) Heatmap of combination index (CI) values at different D: Q ratios and fraction affected (Fa) levels, showing synergistic effects of Dasatinib and Quercetin (DQ). B ) Cell viability curves of normal BMSCs, senescent BMSCs (Sn-BMSCs), and Sn-BMSCs under hypoxia treated with vehicle, free DQ, Asp 10 SAC4A, or DQ@Asp 10 SAC4A. C ) Representative SA-β-Gal staining images of senescent cells treated with different formulations under normoxic and hypoxic conditions, and quantification of SA-β-Gal-positive area (%) ( n = 4/group). Scale bar: 100 μm. D–F ) Representative Western blot images ( D ) and quantitative analyses ( E , F ) of senescence markers P16 and P21 expression in different treatment groups ( n = 3/group). G ) Representative alkaline phosphatase (ALP, upper panel) and Alizarin Red staining (ARS, lower panel) images demonstrating osteogenic differentiation after indicated treatments under normoxic and hypoxic conditions. H–J ) Representative Western blot images ( H ) and quantitative analyses ( I , J ) showing protein expression levels of osteogenic markers RUNX2 and osteopontin (OPN) ( n = 3/group). (Data are presented as mean ± SD; * P < 0.05, ** P < 0.01, *** P < 0.001; n = 3–4/group)

Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from Servicebio (Catalog number STCC6011P).

Techniques: In Vitro, Staining, Western Blot, Expressing

Journal: Journal of Orthopaedic Translation

Article Title: Osteostaticytes: A novel osteoclast subset couples bone resorption and bone formation

doi: 10.1016/j.jot.2024.06.010

Figure Lengend Snippet: Cells at the earlier stage of osteoclast lineage contribute to MSC recruitment A) Schematic diagram of the Transwell assay. MSCs were seeded in the upper well, and osteoclast lineage cells were seeded in the lower well; B) Transwell migration assay of each osteoclast lineage at different time points; C) Analysis of the number of migrated MSCs when cocultured with different osteoclast lineage cells (from day 1 to day 6); Scale bars: 200 μm; D) Schematic diagram of the coculture assay. MSCs were seeded in the upper well, and osteoclast lineage cells were seeded in the lower well; E) Representative images of ALP staining (panoramic and local) of MSCs under osteogenic and adipogenic induction. Scale bars: 200 μm (upper), 50 μm (bottom). F) Quantification of integrated optical density (IOD) for ALP staining for the six groups (n = 3). All data are shown as the mean ± SD. **P ≤ 0.01, *P < 0.05; NS, not significant (P > 0.05).

Article Snippet: Mouse bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and grown in DMEM (Gibco, Carlsbad, California, United States).

Techniques: Transwell Assay, Transwell Migration Assay, Co-culture Assay, Staining

Journal: Journal of Orthopaedic Translation

Article Title: Osteostaticytes: A novel osteoclast subset couples bone resorption and bone formation

doi: 10.1016/j.jot.2024.06.010

Figure Lengend Snippet: OSCs are sufficient for the occurrence of bone mesenchymal stem cells during bone remodeling A) Schematic diagram of the tibia fracture model. B) Representative radiographs of the mouse fracture model via X-ray. C) USA300/Eno-Antares2 tracing bacteria showed the infection condition of fracture lesions every 4 days. D) Bar plot of infection conditions among each time point with USA300/Eno-Antares2 tracing bacteria. All data are shown as the mean ± SD, n = 5. **P ≤ 0.01, *P < 0.05; NS, not significant (P > 0.05). E, F and G) Immunofluorescence staining of IDO1 (red) and CD90 (purple) in normal fracture lesions and infected fracture lesions were stained in sections; E for 2 weeks after fracture, F for 3 weeks after fracture, G for 4 weeks after fracture; Scale bars: 50 μm. H and I) The violin graph shows the statistical results of purple fluorescence intensity (CD90-AF647 nm) representing the number of MSCs (H) and red fluorescence intensity (IDO1-AF568 nm) representing the number of OSCs (I). (n = 5) P values are displayed over the data. BF represents the normal fracture group, and OBF represents the infected fracture group.

Article Snippet: Mouse bone marrow mesenchymal stem cells (BMSCs) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and grown in DMEM (Gibco, Carlsbad, California, United States).

Techniques: Bacteria, Infection, Immunofluorescence, Staining, Fluorescence

Journal: BMC Musculoskeletal Disorders

Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

doi: 10.1186/s12891-024-07236-0

Figure Lengend Snippet: Identification of BMSCs-exosomes. (A) Size distribution of BMSCs-exosomes. (B) transmission electron microscopy image of BMSCs-exosomes. Scale bars: 100 nm. (C) Western blot results of exosome surface proteins

Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

Techniques: Transmission Assay, Electron Microscopy, Western Blot

Journal: BMC Musculoskeletal Disorders

Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

doi: 10.1186/s12891-024-07236-0

Figure Lengend Snippet: Effects of BMSCs-exosomes on the function of MC3T3-E1 cells. (A) Screening of BMSCs-Exo concentration. (B) BMSCS-EXO-induced KLF3-AS1 elevation can be transfected with si-KLF3-AS1 inversion. BMSCs-Exo can promote the (C) cell viability and (D) migration of MC3T3-E1 cells and inhibit (E) cell apoptosis. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group; ## p < 0.01, ### p < 0.001 vs. BMSCs-Exo group ( n = 5, one-way ANOVA).

Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

Techniques: Concentration Assay, Transfection, Migration, Control

Journal: BMC Musculoskeletal Disorders

Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

doi: 10.1186/s12891-024-07236-0

Figure Lengend Snippet: Effects of miR-338-3p in MC3T3-E1 cells incubated with BMSCs-Exo. (A) Transfection of miR-338-3p inhibitors down-regulated the expression level of miR-338-3p in BMSCs-Exo incubated MC3T3-E1 cells. The decrease of miR-338-3p can promote the (B) viability and (C) migration of BMSCs-Exo incubated MC3T3-E1 cells, and inhibit (D) cell apoptosis. *** p < 0.001 vs. Control group; ## p < 0.01, ### p < 0.001 vs. BMSC-Exos + si-NC group; & p < 0.05, && p < 0.01, &&& p < 0.001 vs. BMSC-Exos + si-KLF3-AS1 + inhibitor-NC group ( n = 5, one-way ANOVA).

Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

Techniques: Incubation, Transfection, Expressing, Migration, Control

Journal: BMC Musculoskeletal Disorders

Article Title: Bone marrow mesenchymal stem cell-derived exosomes promote osteoblast proliferation, migration and inhibit apoptosis by regulating KLF3-AS1/miR-338-3p

doi: 10.1186/s12891-024-07236-0

Figure Lengend Snippet: PTEN is a target gene of miR-338-3p. (A) Complementary sites of miR-338-3p and PTEN. (B) luciferase reporter gene assay. *** p < 0.001 vs. Control group ( n = 5, one-way ANOVA). The expression level of PTEN in serum was down-regulated in patients with (C) hand fracture and (D) intraarticular fracture (Healthy individuals = 65, Hand fracture = 35, Intra-articular fracture = 30). *** p < 0.001 vs. Healthy individuals (Independent sample t test). (E) The content of PTEN was increased in BMSCs-Exo incubated cells. *** p < 0.001 vs. control group; ### p < 0.001 vs. BMSCs-Exo + si-NC group; &&& p < 0.001 vs. BMSCs-Exo + si-KLF3-AS1 + inhibitor-NC group ( n = 5, one-way ANOVA).

Article Snippet: Primary mouse bone marrow mesenchymal stem cells (BMSCs) were purchased from ATCC and cultured in a humidity-adjustable incubator containing 5% CO 2 at 37°C.

Techniques: Luciferase, Reporter Gene Assay, Control, Expressing, Incubation